CRISPR:Article Title: The growth factor EPIREGULIN promotes basal progenitor cell proliferation in the developing neocortex.
Article Snippet: .. HiFi Cas9 Nuclease V3 Integrated DNA Technologies Cat. # 1081060 Alt-R CRISPR-Cas9 tracrRNA Integrated DNA Technologies Cat. # 1072533 Recombinant Human EPIREGULIN Protein 1195-EP (without carrier) R&D Systems Cat. # 1195-EP-025/CF Dimethyl sulfoxide (DMSO) Sigma Aldrich Cat. # D2650-100ML AG-1478 (Synonyms: Tyrphostin AG-1478; NSC 693255) BIOZOL Diagnostica Vertrieb GmbH Cat. # MCE-HY-13524 Dacomitinib BIOZOL Diagnostica Vertrieb GmbH Cat. # MCE-HY-13272 Rat serum Charles River Laboratories Japan Crl:CD(SD) Cellmatrix type I-A Kyowa chemical products Cat. # 631-00651 DMEM/F12, HEPES Gibco Cat. # 31330095 Poly-D-Lysine Invitrogen Cat. # A3890401 Laminin from Engelbreth–Holm–Swarm murine sarcoma basement membrane Sigma Aldrich Cat. # L2020 Bovine serum albumin (BSA) Sigma Aldrich Cat. # A2153 Fetal bovine serum (FBS) Sigma Aldrich Cat. # F7524 Heparin sodium salt from porcine intestinal mucosa Sigma Aldrich Cat. # H4784 MEM Non-essential amino acids solution (100X) Gibco Cat. # 11140050 GlutaMAX Supplement Gibco Cat. # 35050038 Human EGF Recombinant Protein Gibco Cat. # PHG0311 14 The EMBO Journal © The Author(s) on A pril 10, 2024 from IP 3.215.103.70 ..
Recombinant:Article Title: The growth factor EPIREGULIN promotes basal progenitor cell proliferation in the developing neocortex.
Article Snippet: .. HiFi Cas9 Nuclease V3 Integrated DNA Technologies Cat. # 1081060 Alt-R CRISPR-Cas9 tracrRNA Integrated DNA Technologies Cat. # 1072533 Recombinant Human EPIREGULIN Protein 1195-EP (without carrier) R&D Systems Cat. # 1195-EP-025/CF Dimethyl sulfoxide (DMSO) Sigma Aldrich Cat. # D2650-100ML AG-1478 (Synonyms: Tyrphostin AG-1478; NSC 693255) BIOZOL Diagnostica Vertrieb GmbH Cat. # MCE-HY-13524 Dacomitinib BIOZOL Diagnostica Vertrieb GmbH Cat. # MCE-HY-13272 Rat serum Charles River Laboratories Japan Crl:CD(SD) Cellmatrix type I-A Kyowa chemical products Cat. # 631-00651 DMEM/F12, HEPES Gibco Cat. # 31330095 Poly-D-Lysine Invitrogen Cat. # A3890401 Laminin from Engelbreth–Holm–Swarm murine sarcoma basement membrane Sigma Aldrich Cat. # L2020 Bovine serum albumin (BSA) Sigma Aldrich Cat. # A2153 Fetal bovine serum (FBS) Sigma Aldrich Cat. # F7524 Heparin sodium salt from porcine intestinal mucosa Sigma Aldrich Cat. # H4784 MEM Non-essential amino acids solution (100X) Gibco Cat. # 11140050 GlutaMAX Supplement Gibco Cat. # 35050038 Human EGF Recombinant Protein Gibco Cat. # PHG0311 14 The EMBO Journal © The Author(s) on A pril 10, 2024 from IP 3.215.103.70 ..
Article Title: Investigation of synaptic connectivity in functional in vitro neuronal assemblies.
Article Snippet: .. The procedures were approved by local veterinary authorities and the Italian REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies RFP 1:1000 Rockland Cat. No: 600-401-379; RRID: AB_2209751 HA 1:2000 Abcam Cat. No: ab9111; RRID:AB_307020 cFos 1:1000 Synaptic Systems Cat. No: 226008; RRID:AB_2891278 MAP2 1:1000 Covance Cat. No: SMI-52P; RRID:AB_2728526 SMI 312 1:1000 Covance Cat. No. SMI-312R; RRID:AB_2314906 PSD95 1:1000 Millipore Cat. No: #MAB1596; RRID: AB_2092365 GFAP 1:500 Alomone Cat. No: AGP-307 vGlut1 1:500 Thermo Fisher Scientific Cat. No: PA5-85764; RRID:AB_2792901 Bacterial and virus strains AAV9-hSyn-hChR2(H134R)-EYFP Addgene Addgene viral prep # 26973-AAV9; RRID: Addgene_26973 Chemicals, peptides, and recombinant proteins TTX Tocris Cat. No.:#1069 Doxycicline Merck Cat. No.:#D9891 HBSS Gibco Cat. No.:#14175053 HEPES Gibco Cat. No.:#15630049 PBS Gibco Cat.No. .. :#70011036 Trypsin/EDTA Gibco Cat. No.:#25200072 DMEM Gibco Cat. No.:#11885084 penicillin-streptomycin 10000 U/ml Gibco Cat. No.:#15140163 Poli-L-lysine Merck Millipore Cat. No.:#P1399 neurobasal Gibco Cat. No.:#21103049 B27 Gibco Cat. No.:#17504044 Glutamax Gibco Cat. No.:#35050061 DAPI Invitrogen Cat. No.:#D1306 Aqua Polymount Polyscience Inc. Cat. No.:#18606 x-rhod-1 a.m. Invitrogen Cat. No.:#X14210 Experimental models: Organisms/strains Mice/C57Bl6J Charles Rivers N/A Recombinant DNA TreP-Arc3 ′ -Nend-PSDTag-Venus-HAArc5 ′ UTR Prof. A. Cattaneo; Gobbo et al. 11 N/A CAG-rtTA-IRES-TdTomato Prof. A. Cattaneo; Gobbo et al. 11 N/A pAAV.CAG.hChR2(H134R)mCherry.WPRE.SV40 Addgene Addgene plasmid # 100054; http://n2t.net/ addgene:100054; RRID:Addgene_100054); Software and algorithms Nis Element Nikon N/A Prism GraphPad N/A MATLAB MathWorks N/A e1 Cell Reports Methods 6, 101265, January 26, 2026 Ministry of Health (3FAF3.N.1JY).
Article Title: IGF2BP1 restricts the induction of human primordial germ cell fate in an m 6 A-dependent manner.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Matrigel Corning Cat#354234 Nuwacell® ncTarget hPSC Medium Nuwacell Cat#RP01020 Primocin InvitroGen Cat#ant-pm-2 Dispase STEMCELL TM Technologies Cat#07923 GibcoTM BASIC DMEM/F-12, HEPES GIBCO CatC11330500BT KnockOut Serum GIBCO Cat#10828028 non-essential amino acids GIBCO Cat#11140-050 β-mercaptoethanol GIBCO Cat#21985023 Penicillin-Streptomycin-Glutamine Invitrogen Cat#10378016 ACCUTASE STEMCELL TM Technologies Cat#07992 Human plasma fibronectin Invitrogen Cat#33016015 sodium pyruvate GIBCO Cat#11360070 FGF2 Peprotech Cat#100-18B ACTA Peprotech Cat#AF120-14E CHIR99021 Stemgent Cat#04000410 Y-27632 (Selleck, S1049) Selleck Cat#S1049 Glasgow’s MEM GIBCO Cat#11710-035 Anti-Adherence Rinsing Solution STEMCELL TM Technologies Cat#07010 LIF PeproTech Cat#300-05-25 BMP4 R&D Systems Cat#314-BP-01M EGF R&D Systems Cat#236-EG-01M T4 enzyme New England Biolabs Cat#M0202L BbSI enzyme New England Biolabs Cat#R3539 Puromycin Selleck Cat#S7417 Blasticidin Solarbio Cat#B9300 Ganciclovir MCE Cat#82410-32-0 PEI Polysciences Cat#24765100 PEG 8000 Solarbio Cat#P8260 0.05% trypsin GIBCO Cat#25300054 7AAD Beyotime Cat#ST515 4% paraformaldehyde Biosharp Cat#BL539A DAPI Beyotime Cat#C1002 AG RNAex Pro Reagent Accurate Biology Cat#AG21101 Actinomycin D Sigma-Aldrich Cat#SBR00013 RNasin Plus RNase Inhibitor Promega Cat#N2611 Cocktail protease inhibitors Roche N/A Protein A/G magnetic beads Thermo Fisher Scientific Cat#88802 FLAG M2 magnetic beads Sigma-Aldrich Cat#A2220 Proteinase K Thermo Fisher Scientific Cat#EO0491 TRIzol Invitrogen Cat#15596026 VAHTS mRNA Capture Beads Vazyme Cat#N401 IGEPAL® CA-630 Sigma-Aldrich Cat#56741 Protease inhibitors Sigma-Aldrich Cat#1187358001 Iodoacetamide Sigma-Aldrich Cat#I1149 Trypsin Promega Cat#V5280 TFA Sigma-Aldrich Cat#1002641000 RIPA buffer Beyotime Cat#P0013C Aprotinin Solarbio Cat#IA0430 Leupeptin Solarbio Cat#IL0070 (Continued on next page) Cell Stem Cell 32, 1–17.e1–e13, July 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Pepstatin Solarbio Cat#IP0170 HA beads Smart-Lifesciences Cat#SA068001 Ni-charged magnetic beads Engibody Cat#IF9301 nuclease P1 Wako Cat#145-08221 Shrimp alkaline phosphatase NEB Cat#M0371V Critical commercial assays STEMdiff TM Mesoderm Induction Medium Kit STEMCELL TM Technologies Cat#05220 STEMdiff TM Definitive Endoderm Medium Kit STEMCELL TM Technologies Cat#05110 STEMdiff TM Neural induction medium Kit STEMCELL TM Technologies Cat#08581 Phanta Max Super-Fidelity DNA Polymerase Vazyme Cat#PD505 ClonExpress MultiS One Step Cloning Kit Vazyme Cat#C11302 ClonExpressII One Step Cloning Kit Vazyme Cat#C11202 P3 Primary Cell 4D-Nucleofector X Kit Lonza Cat#V4XP-3024 EdU assay kit Sangon Biotech Cat#E6072040100 Dual Luciferase Reporter Gene Assay Kit Beyotime Cat#RG028 Western-Bright ECL kit Advansta Cat#K12045D50 mMESSAGE mMACHINE® SP6 Kit Invitrogen/ambion Cat#AM1340 Picoliter microinjection system WARNER Cat#PLI-100A Fast RNA-seq Lib Prep Kit V2 ABclonal Cat#RK20306 RNeasy Micro Kit QIAGEN Cat#4004 VAHTS universal V8 RNA-seq library Prep Kit Vazyme Cat#NR605 SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian Takara-Clontech Cat#634413 Hyperactive Universal CUT&Tag Assay Kit for Illumina Vazyme Cat#TD903 RNA clean&ConcentratorTM-5 kit Zymo Cat#R1014 Deposited data wild type_hESCs_rep1 This study GSE266418 wild type_hESCs_rep2 This study GSE266418 IGF2BP1-KO_hESCs_rep1 This study GSE266418 IGF2BP1-KO_hESCs_rep2 This study GSE266418 OTX2-KO_hESCs_rep1 This study GSE266418 OTX2-KO_hESCs_rep2 This study GSE266418 OTX2-GFP-OE_hESCs_rep1 This study GSE266418 OTX2-GFP-OE_hESCs_rep2 This study GSE266418 wild type_endoderm_rep1 This study GSE266418 wild type_endoderm_rep2 This study GSE266418 IGF2BP1-KO_endoderm_rep1 This study GSE266418 IGF2BP1-KO_endoderm_rep2 This study GSE266418 wild type_mesoderm_rep1 This study GSE266418 wild type_mesoderm_rep2 This study GSE266418 IGF2BP1-KO_mesoderm_rep1 This study GSE266418 IGF2BP1-KO_mesoderm_rep2 This study GSE266418 wild type_ectoderm_rep1 This study GSE266418 wild type_ectoderm_rep2 This study GSE266418 IGF2BP1-KO_ectoderm_rep1 This study GSE266418 IGF2BP1-KO_ectoderm_rep2 This study GSE266418 wild type_iMeLCs_rep1 This study GSE266418 wild type_iMeLCs_rep2 This study GSE266418 IGF2BP1-KO_iMeLCs_rep1 This study GSE266418 (Continued on next page) e3 Cell Stem Cell 32, 1–17.e1–e13, July 3, 2025
Membrane:Article Title: The growth factor EPIREGULIN promotes basal progenitor cell proliferation in the developing neocortex.
Article Snippet: .. HiFi Cas9 Nuclease V3 Integrated DNA Technologies Cat. # 1081060 Alt-R CRISPR-Cas9 tracrRNA Integrated DNA Technologies Cat. # 1072533 Recombinant Human EPIREGULIN Protein 1195-EP (without carrier) R&D Systems Cat. # 1195-EP-025/CF Dimethyl sulfoxide (DMSO) Sigma Aldrich Cat. # D2650-100ML AG-1478 (Synonyms: Tyrphostin AG-1478; NSC 693255) BIOZOL Diagnostica Vertrieb GmbH Cat. # MCE-HY-13524 Dacomitinib BIOZOL Diagnostica Vertrieb GmbH Cat. # MCE-HY-13272 Rat serum Charles River Laboratories Japan Crl:CD(SD) Cellmatrix type I-A Kyowa chemical products Cat. # 631-00651 DMEM/F12, HEPES Gibco Cat. # 31330095 Poly-D-Lysine Invitrogen Cat. # A3890401 Laminin from Engelbreth–Holm–Swarm murine sarcoma basement membrane Sigma Aldrich Cat. # L2020 Bovine serum albumin (BSA) Sigma Aldrich Cat. # A2153 Fetal bovine serum (FBS) Sigma Aldrich Cat. # F7524 Heparin sodium salt from porcine intestinal mucosa Sigma Aldrich Cat. # H4784 MEM Non-essential amino acids solution (100X) Gibco Cat. # 11140050 GlutaMAX Supplement Gibco Cat. # 35050038 Human EGF Recombinant Protein Gibco Cat. # PHG0311 14 The EMBO Journal © The Author(s) on A pril 10, 2024 from IP 3.215.103.70 ..
other:Article Title: Sialylated IgG induces the transcription factor REST in alveolar macrophages to protect against lung inflammation and severe influenza disease.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Phosphate buffered saline Gibco cat. no. 14190-144 Non-fat Milk Lab Scientific bioKEMIX cat. no. M0841 SDS sample buffer Boster cat. no. AR1112 Single-cell lysis buffer Takara cat. no. 635013 RNase inhibitor Takara cat. no. 2313B Fixation Buffer BioLegend cat. no. 420801 DharmaFect3 Transfection Reagent Horizon Discovery cat. no. NC1650394 Non-essential amino acids Gibco cat. no. 11-140-050 Sodium pyruvate Gibco cat. no. 11-360-070 Macrophage-colony stimulating factor (M-CSF) Peprotech cat. no. 300-25 IL-4 Peprotech cat. no. 200-04 glycine Sigma-Aldrich cat. no. G8898 TritonX-100 Sigma-Aldrich cat. no. 93443 ProLong Gold Antifade Reagent Cell Signaling Technology cat. no. P36930 Normocin Invivogen cat. no. ant-nr-1 Blasticidin Invivogen cat. no. ant-bl-1 phorbol 12-myristate 13-acetate (PMA) Thermo Fisher Scientific cat. no. J63916.MCR lipopolysaccharide (LPS) eBioscience cat. no. 50-112-2025 QUANTI-Blue solution Invivogen cat. no. rep-qbs Hoechst 33342 Abcam Inc. cat. no. ab228551 Alexa Fluor 488 phalloidin Invitrogen cat. no. A12379 LIVE/DEAD Blue Fixable Dye Thermo Fisher Scientific cat. no. L34962 LIVE/DEAD Aqua Fixable Dye Thermo Fisher Scientific cat. no. L34957 Critical Commercial Assays Nano-Glo Luciferase Assay System Promega cat. no. N1120 Legendplex Bead array kits Biolegend cat. nos.
Article Title: Reprogramming of endolysosomes for melanogenesis in BLOC-1-deficient melanocytes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Luna Universal qPCR Master Mix New England Biolabs Cat. # M3003 Experimental models: Cell lines melan-pa: Bloc1S6 pa/pa Functional Genomics Cell Bank at City St. George’s University of London N/A melan-mu: Bloc1S5 mu/mu Functional Genomics Cell Bank at City St. George’s University of London N/A melan-a: a/a Functional Genomics Cell Bank at City St. George’s University of London N/A melan-Ink4a-Arf1: Ink4a-Arf -/- Functional Genomics Cell Bank at City St. George’s University of London N/A melan-c2: Tyr c-2J /Tyr c-2J Functional Genomics Cell Bank at City St. George’s University of London N/A melan-mu:MuHA Functional Genomics Cell Bank at City St. George’s University of London N/A Oligonucleotides TYR Fwd: 5 ’ CGA TGG AAC ACC TGA GGG AC Integrated DNA Technologies N/A TYR Rev:5 ’ AGT GGA CTG GCA AAT CCT TCC Integrated DNA Technologies N/A Atp5b primer set (Anchor nucelotide: 115) Primer Design Ltd. geNorm kit (Discontinued) Ubc primer set (Anchor nucelotide: 2627) Primer Design Ltd. geNorm kit (Discontinued) B2m primer set (Anchor nucelotide: 202) Primer Design Ltd. geNorm kit (Discontinued) Sdha primer set (Anchor nucelotide: 2018) Primer Design Ltd. geNorm kit (Discontinued) Recombinant DNA pCR3/OCA2-HA Michael Marks N/A pCI-HA-SLC45A2 Michael Marks N/A pN1-LAMP1-tdTomato Tom Carter N/A Software and algorithms GraphPad Prism (version 10.2.1) GraphPad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ij/ Photoshop CS6 (Version 13.0) Adobe Systems N/A NIS Elements (Version 4.2) Nikon N/A Image Studio (Version 5.2) Li-Cor N/A AMT Capture Engine software (Version V602.580.20d) Advanced Microscopy Techniques N/A Other RPMI-1640 Medium Sigma-Alrich Cat. # R7509 Fetal Bovine Serum Gibco Cat. # 10270106 Dextran, Oregon Green 488; 10,000 MW Invitrogen Cat. # D7170 L-Glutamine Gibco Cat. # 25030081 Penicillin-Streptomycin (10,000 U/mL) Gibco Cat. # 15140122 HEPES Gibco Cat. # 15630056 Protease Inhibitor cocktail Roche Cat. # 11697498001 Immobilon-FL PVDF Membrane Millipore Cat. # IPFL85R Nitrocellulose membrane, 0.45 μm BioRad Cat. # 1620115 CitiFluor AF1 mounting solution Agar Scientific Cat. # AGR1320 Copper mesh grids Agar Scientific Cat. # AGG2415C 35 mm Dish with No. 1.5 Gridded Coverslip, 14 mm Glass Diameter MatTek Cat. # P35G-1.5-14-C-GRD CitiFluor AF1 mounting solution Agar Scientific Cat. #AGR1320 Black 96 well plate Greiner Bio-One Cat. #655900 e2 Current Biology 35, 3570–3586.e1–e7, August 4, 2025
Article Title: Canonical and non-canonical PRC1 differentially contribute to regulation of neural stem cell fate.
Article Snippet: PRC1 function in neural stem cells Hoffmann et al. https://doi.org/10.26508/lsa.202403006 vol 8 | no 4 | e202403006 7 of 16 Continued Reagent/resource Reference or source Identifier or catalogue number Mouse anti-Mel18 (IF 1:50, WB 1:200) SC-515329; Santa Cruz RRID: AB_2687587 Mouse anti-Bmi-1 (IF 1:300; WB 1:1,000) 05-637; EMD Millipore RRID: AB_309865 Chicken anti-Nestin (IF 1:500) ab134017; Abcam RRID: AB_2753197 Mouse anti-HuC/HuD (IF 1:300) A21271; Invitrogen RRID: AB_221448 Rat anti-GFAP (IF 1:500) 13-0300; Invitrogen RRID: AB_2532994 Rabbit anti-PDGFR alpha (IF 1:500) ab203491; Abcam RRID: AB_2893014 Mouse anti-APC (CC-1, IF 1:500) OP80; Merck RRID: AB_2057371 Rabbit anti-PCGF3/5 (WB 1:1,000) ab201510; Abcam RRID: AB_2818981 Mouse anti-vinculin (WB 1:1,000) V9131; Sigma-Aldrich RRID: AB_477629 Donkey anti-goat IgG, Alexa Fluor 488 conjugated (1:1,000) A-11055; Invitrogen RRID: AB_2534102 Goat anti-rat IgG, Alexa Fluor 633 conjugated (1:1,000) A-21094; Invitrogen RRID: AB_2535731 Donkey anti-mouse IgG, Alexa Fluor 555 conjugated (1:1,000) A-31570; Invitrogen RRID: AB_2536180 Donkey anti-rabbit IgG, Alexa Fluor 555 conjugated (1:1,000) A-31572; Invitrogen RRID: AB_2536182 Goat anti-chicken IgG, Alexa Fluor 488 conjugated (1:1,000) A-11039; Invitrogen RRID: AB_2534096 Donkey anti-rat IgG, Alexa Fluor 555 conjugated (1:1,000) A-48270; Invitrogen RRID: AB_2536114 Donkey anti-mouse IgG, Alexa Fluor 647 conjugated (1:1,000) A-31571; Invitrogen RRID: AB_162542 Donkey anti-rabbit IgG, Alexa Fluor 647 conjugated (1:1,000) A-31573; Invitrogen RRID: AB_2536183 Goat anti-rabbit IgG, H&L HRP (1:3,000) ab6721; Abcam RRID: AB_955447 Goat anti-mouse IgG, H&L HRP (1:10,000) ab205719; Abcam RRID: AB_2755049 Oligonucleotides Primers This study Table S1 Chemicals, enzymes, and other reagents DMEM/F-12, Hepes Gibco Cat. # 31330095 Poly-D-lysine Invitrogen Cat. # A3890401 Laminin from the Engelbreth–Holm–Swarm murine sarcoma basement membrane Sigma-Aldrich Cat. # L2020 BSA Sigma-Aldrich Cat. # A2153 FBS Sigma-Aldrich Cat. # F7524 Heparin sodium salt from porcine intestinal mucosa Sigma-Aldrich Cat. # H4784 MEM Non-essential Amino Acid Solution (100X) Gibco Cat. # 11140050 GlutaMAX supplement Gibco Cat. # 35050038 Human EGF Recombinant Protein Gibco Cat. # PHG0311 Human FGF-basic (FGF-2/bFGF) (aa 10-155) Recombinant Protein Gibco Cat. # PHG0021 B-27 supplement (50x), serum-free Gibco Cat. # 17504044 N-2 supplement (100X) Gibco Cat. # 17502048 OH-tamoxifen Sigma-Aldrich Cat. # H7904 Thermo Fisher Scientific Halt Protease Inhibitor Cocktail (100x) Thermo Fisher Scientific Cat. # 10516495 (Continued on following page) PRC1 function in neural stem cells Hoffmann et al. https://doi.org/10.26508/lsa.202403006 vol 8 | no 4 | e202403006 8 of 16
Article Title: Deep-learning-based three-dimensional label-free tracking and analysis of immunological synapses of CAR-T cells
Article Snippet: DOI: https://doi.org/10.7554/eLife.49023 16 of 26 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Recombinant DNA reagent pMACS LNGFR Miltenyi Biotec Cat. #:130-091-890 Peptide, recombinant protein rhIL-2 BMI KOREA 300IU/mL Peptide, recombinant protein rhCD19-Fc ACRO Biosystems Cat. #: CD9-H5259 FACS Peptide, recombinant protein AF647-conjugated streptavidin Biolegend Cat. #: 405237 FACS Antibody InVivoMab anti-human CD3 Bio X cell Cat. #: BE0001-2-5MG OKT3 clone 4 mg/mL coated Antibody InVivoMab anti-human CD28 Bio X cell Cat. #: BE0291-5MG CD28.2 clone 2 mg/mL soluble Antibody hLNGFR-APC antibody Miltenyi Biotec Cat. #: 130-113-418 FACS Antibody hCD19-APC antibody Biolegend Cat. #: 302212 FACS Other DPBS Welgene Cat. #: LB001-02 Other Fetal Bovine Serum (FBS) Gibco Cat. #: 26140–079 Other DMEM Gibco Cat. #: 11965–118 Other RPMI Gibco Cat. #: 21870–092 Other HBSS with Ca2+ and Mg2+ Gibco Cat #: 14-025-092 Other HEPES Gibco Cat. #: 1530080 Other 2-Mercaptoethanol Sigma Cat. #: M6250-100mL Other Lipofectamine 2000 ThermoFisher Cat. #: 11668019 Other Glutamax Gibco Cat. #: 35050–061 Other Protamine Sulfate Sigma Cat. #: P3369 Other MEM Non-essential amino acid Gibco Cat. #: 111400500 Other Sodium pyruvate ThermoFisher Cat. #: 11360070 Other penicillin/streptomycin Gibco Cat. #: 15140–122 Other Tomodish Tomocube Other Paraformaldehyde Solution, 4% in PBS ThermoFisher Cat. #: AAJ19943K2 Fixation Other CellBrite Fix 640 Membrane Dye Biotium Cat. #: 30089 Plasma membrane staining Other VECTASHIELD Hardset w/ DAPI VECTOR Laboratory Cat. #: H-1400 Mounting Medium Other LysoTracker Deep Red ThermoFisher Cat #: L12492 Dye for labeling and tracking lytic granules Other TetraSpeck Microspheres,0.1 mm, fluorescent blue/green/ orange/dark red ThermoFisher Cat #: T7279 Fiducial markers Other FITC-dextran 10 kDa TdB Labs Cat #: 20682 Fluorescein-labeled dextran, 10 kDa Other FITC-dextran 2000 kDa TdB Labs Cat #: 20584 Fluorescein-labeled dextran, 2000 kDa Commercial assay or kit CD271 Microbeads kits, Human Miltenyi Biotec Cat. #: 130-099-023 Isolation of CAR+ T cells Commercial assay or kit SepMate PBMC Isolation tube STEMCELL 86460 PBMC Isolation Continued on next page Lee, Lee, Song, et al. eLife 2020;9:e49023.
Virus:Article Title: Investigation of synaptic connectivity in functional in vitro neuronal assemblies.
Article Snippet: .. The procedures were approved by local veterinary authorities and the Italian REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies RFP 1:1000 Rockland Cat. No: 600-401-379; RRID: AB_2209751 HA 1:2000 Abcam Cat. No: ab9111; RRID:AB_307020 cFos 1:1000 Synaptic Systems Cat. No: 226008; RRID:AB_2891278 MAP2 1:1000 Covance Cat. No: SMI-52P; RRID:AB_2728526 SMI 312 1:1000 Covance Cat. No. SMI-312R; RRID:AB_2314906 PSD95 1:1000 Millipore Cat. No: #MAB1596; RRID: AB_2092365 GFAP 1:500 Alomone Cat. No: AGP-307 vGlut1 1:500 Thermo Fisher Scientific Cat. No: PA5-85764; RRID:AB_2792901 Bacterial and virus strains AAV9-hSyn-hChR2(H134R)-EYFP Addgene Addgene viral prep # 26973-AAV9; RRID: Addgene_26973 Chemicals, peptides, and recombinant proteins TTX Tocris Cat. No.:#1069 Doxycicline Merck Cat. No.:#D9891 HBSS Gibco Cat. No.:#14175053 HEPES Gibco Cat. No.:#15630049 PBS Gibco Cat.No. .. :#70011036 Trypsin/EDTA Gibco Cat. No.:#25200072 DMEM Gibco Cat. No.:#11885084 penicillin-streptomycin 10000 U/ml Gibco Cat. No.:#15140163 Poli-L-lysine Merck Millipore Cat. No.:#P1399 neurobasal Gibco Cat. No.:#21103049 B27 Gibco Cat. No.:#17504044 Glutamax Gibco Cat. No.:#35050061 DAPI Invitrogen Cat. No.:#D1306 Aqua Polymount Polyscience Inc. Cat. No.:#18606 x-rhod-1 a.m. Invitrogen Cat. No.:#X14210 Experimental models: Organisms/strains Mice/C57Bl6J Charles Rivers N/A Recombinant DNA TreP-Arc3 ′ -Nend-PSDTag-Venus-HAArc5 ′ UTR Prof. A. Cattaneo; Gobbo et al. 11 N/A CAG-rtTA-IRES-TdTomato Prof. A. Cattaneo; Gobbo et al. 11 N/A pAAV.CAG.hChR2(H134R)mCherry.WPRE.SV40 Addgene Addgene plasmid # 100054; http://n2t.net/ addgene:100054; RRID:Addgene_100054); Software and algorithms Nis Element Nikon N/A Prism GraphPad N/A MATLAB MathWorks N/A e1 Cell Reports Methods 6, 101265, January 26, 2026 Ministry of Health (3FAF3.N.1JY).
Knock-Out:Article Title: IGF2BP1 restricts the induction of human primordial germ cell fate in an m 6 A-dependent manner.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Matrigel Corning Cat#354234 Nuwacell® ncTarget hPSC Medium Nuwacell Cat#RP01020 Primocin InvitroGen Cat#ant-pm-2 Dispase STEMCELL TM Technologies Cat#07923 GibcoTM BASIC DMEM/F-12, HEPES GIBCO CatC11330500BT KnockOut Serum GIBCO Cat#10828028 non-essential amino acids GIBCO Cat#11140-050 β-mercaptoethanol GIBCO Cat#21985023 Penicillin-Streptomycin-Glutamine Invitrogen Cat#10378016 ACCUTASE STEMCELL TM Technologies Cat#07992 Human plasma fibronectin Invitrogen Cat#33016015 sodium pyruvate GIBCO Cat#11360070 FGF2 Peprotech Cat#100-18B ACTA Peprotech Cat#AF120-14E CHIR99021 Stemgent Cat#04000410 Y-27632 (Selleck, S1049) Selleck Cat#S1049 Glasgow’s MEM GIBCO Cat#11710-035 Anti-Adherence Rinsing Solution STEMCELL TM Technologies Cat#07010 LIF PeproTech Cat#300-05-25 BMP4 R&D Systems Cat#314-BP-01M EGF R&D Systems Cat#236-EG-01M T4 enzyme New England Biolabs Cat#M0202L BbSI enzyme New England Biolabs Cat#R3539 Puromycin Selleck Cat#S7417 Blasticidin Solarbio Cat#B9300 Ganciclovir MCE Cat#82410-32-0 PEI Polysciences Cat#24765100 PEG 8000 Solarbio Cat#P8260 0.05% trypsin GIBCO Cat#25300054 7AAD Beyotime Cat#ST515 4% paraformaldehyde Biosharp Cat#BL539A DAPI Beyotime Cat#C1002 AG RNAex Pro Reagent Accurate Biology Cat#AG21101 Actinomycin D Sigma-Aldrich Cat#SBR00013 RNasin Plus RNase Inhibitor Promega Cat#N2611 Cocktail protease inhibitors Roche N/A Protein A/G magnetic beads Thermo Fisher Scientific Cat#88802 FLAG M2 magnetic beads Sigma-Aldrich Cat#A2220 Proteinase K Thermo Fisher Scientific Cat#EO0491 TRIzol Invitrogen Cat#15596026 VAHTS mRNA Capture Beads Vazyme Cat#N401 IGEPAL® CA-630 Sigma-Aldrich Cat#56741 Protease inhibitors Sigma-Aldrich Cat#1187358001 Iodoacetamide Sigma-Aldrich Cat#I1149 Trypsin Promega Cat#V5280 TFA Sigma-Aldrich Cat#1002641000 RIPA buffer Beyotime Cat#P0013C Aprotinin Solarbio Cat#IA0430 Leupeptin Solarbio Cat#IL0070 (Continued on next page) Cell Stem Cell 32, 1–17.e1–e13, July 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Pepstatin Solarbio Cat#IP0170 HA beads Smart-Lifesciences Cat#SA068001 Ni-charged magnetic beads Engibody Cat#IF9301 nuclease P1 Wako Cat#145-08221 Shrimp alkaline phosphatase NEB Cat#M0371V Critical commercial assays STEMdiff TM Mesoderm Induction Medium Kit STEMCELL TM Technologies Cat#05220 STEMdiff TM Definitive Endoderm Medium Kit STEMCELL TM Technologies Cat#05110 STEMdiff TM Neural induction medium Kit STEMCELL TM Technologies Cat#08581 Phanta Max Super-Fidelity DNA Polymerase Vazyme Cat#PD505 ClonExpress MultiS One Step Cloning Kit Vazyme Cat#C11302 ClonExpressII One Step Cloning Kit Vazyme Cat#C11202 P3 Primary Cell 4D-Nucleofector X Kit Lonza Cat#V4XP-3024 EdU assay kit Sangon Biotech Cat#E6072040100 Dual Luciferase Reporter Gene Assay Kit Beyotime Cat#RG028 Western-Bright ECL kit Advansta Cat#K12045D50 mMESSAGE mMACHINE® SP6 Kit Invitrogen/ambion Cat#AM1340 Picoliter microinjection system WARNER Cat#PLI-100A Fast RNA-seq Lib Prep Kit V2 ABclonal Cat#RK20306 RNeasy Micro Kit QIAGEN Cat#4004 VAHTS universal V8 RNA-seq library Prep Kit Vazyme Cat#NR605 SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian Takara-Clontech Cat#634413 Hyperactive Universal CUT&Tag Assay Kit for Illumina Vazyme Cat#TD903 RNA clean&ConcentratorTM-5 kit Zymo Cat#R1014 Deposited data wild type_hESCs_rep1 This study GSE266418 wild type_hESCs_rep2 This study GSE266418 IGF2BP1-KO_hESCs_rep1 This study GSE266418 IGF2BP1-KO_hESCs_rep2 This study GSE266418 OTX2-KO_hESCs_rep1 This study GSE266418 OTX2-KO_hESCs_rep2 This study GSE266418 OTX2-GFP-OE_hESCs_rep1 This study GSE266418 OTX2-GFP-OE_hESCs_rep2 This study GSE266418 wild type_endoderm_rep1 This study GSE266418 wild type_endoderm_rep2 This study GSE266418 IGF2BP1-KO_endoderm_rep1 This study GSE266418 IGF2BP1-KO_endoderm_rep2 This study GSE266418 wild type_mesoderm_rep1 This study GSE266418 wild type_mesoderm_rep2 This study GSE266418 IGF2BP1-KO_mesoderm_rep1 This study GSE266418 IGF2BP1-KO_mesoderm_rep2 This study GSE266418 wild type_ectoderm_rep1 This study GSE266418 wild type_ectoderm_rep2 This study GSE266418 IGF2BP1-KO_ectoderm_rep1 This study GSE266418 IGF2BP1-KO_ectoderm_rep2 This study GSE266418 wild type_iMeLCs_rep1 This study GSE266418 wild type_iMeLCs_rep2 This study GSE266418 IGF2BP1-KO_iMeLCs_rep1 This study GSE266418 (Continued on next page) e3 Cell Stem Cell 32, 1–17.e1–e13, July 3, 2025
Clinical Proteomics:Article Title: IGF2BP1 restricts the induction of human primordial germ cell fate in an m 6 A-dependent manner.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Matrigel Corning Cat#354234 Nuwacell® ncTarget hPSC Medium Nuwacell Cat#RP01020 Primocin InvitroGen Cat#ant-pm-2 Dispase STEMCELL TM Technologies Cat#07923 GibcoTM BASIC DMEM/F-12, HEPES GIBCO CatC11330500BT KnockOut Serum GIBCO Cat#10828028 non-essential amino acids GIBCO Cat#11140-050 β-mercaptoethanol GIBCO Cat#21985023 Penicillin-Streptomycin-Glutamine Invitrogen Cat#10378016 ACCUTASE STEMCELL TM Technologies Cat#07992 Human plasma fibronectin Invitrogen Cat#33016015 sodium pyruvate GIBCO Cat#11360070 FGF2 Peprotech Cat#100-18B ACTA Peprotech Cat#AF120-14E CHIR99021 Stemgent Cat#04000410 Y-27632 (Selleck, S1049) Selleck Cat#S1049 Glasgow’s MEM GIBCO Cat#11710-035 Anti-Adherence Rinsing Solution STEMCELL TM Technologies Cat#07010 LIF PeproTech Cat#300-05-25 BMP4 R&D Systems Cat#314-BP-01M EGF R&D Systems Cat#236-EG-01M T4 enzyme New England Biolabs Cat#M0202L BbSI enzyme New England Biolabs Cat#R3539 Puromycin Selleck Cat#S7417 Blasticidin Solarbio Cat#B9300 Ganciclovir MCE Cat#82410-32-0 PEI Polysciences Cat#24765100 PEG 8000 Solarbio Cat#P8260 0.05% trypsin GIBCO Cat#25300054 7AAD Beyotime Cat#ST515 4% paraformaldehyde Biosharp Cat#BL539A DAPI Beyotime Cat#C1002 AG RNAex Pro Reagent Accurate Biology Cat#AG21101 Actinomycin D Sigma-Aldrich Cat#SBR00013 RNasin Plus RNase Inhibitor Promega Cat#N2611 Cocktail protease inhibitors Roche N/A Protein A/G magnetic beads Thermo Fisher Scientific Cat#88802 FLAG M2 magnetic beads Sigma-Aldrich Cat#A2220 Proteinase K Thermo Fisher Scientific Cat#EO0491 TRIzol Invitrogen Cat#15596026 VAHTS mRNA Capture Beads Vazyme Cat#N401 IGEPAL® CA-630 Sigma-Aldrich Cat#56741 Protease inhibitors Sigma-Aldrich Cat#1187358001 Iodoacetamide Sigma-Aldrich Cat#I1149 Trypsin Promega Cat#V5280 TFA Sigma-Aldrich Cat#1002641000 RIPA buffer Beyotime Cat#P0013C Aprotinin Solarbio Cat#IA0430 Leupeptin Solarbio Cat#IL0070 (Continued on next page) Cell Stem Cell 32, 1–17.e1–e13, July 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Pepstatin Solarbio Cat#IP0170 HA beads Smart-Lifesciences Cat#SA068001 Ni-charged magnetic beads Engibody Cat#IF9301 nuclease P1 Wako Cat#145-08221 Shrimp alkaline phosphatase NEB Cat#M0371V Critical commercial assays STEMdiff TM Mesoderm Induction Medium Kit STEMCELL TM Technologies Cat#05220 STEMdiff TM Definitive Endoderm Medium Kit STEMCELL TM Technologies Cat#05110 STEMdiff TM Neural induction medium Kit STEMCELL TM Technologies Cat#08581 Phanta Max Super-Fidelity DNA Polymerase Vazyme Cat#PD505 ClonExpress MultiS One Step Cloning Kit Vazyme Cat#C11302 ClonExpressII One Step Cloning Kit Vazyme Cat#C11202 P3 Primary Cell 4D-Nucleofector X Kit Lonza Cat#V4XP-3024 EdU assay kit Sangon Biotech Cat#E6072040100 Dual Luciferase Reporter Gene Assay Kit Beyotime Cat#RG028 Western-Bright ECL kit Advansta Cat#K12045D50 mMESSAGE mMACHINE® SP6 Kit Invitrogen/ambion Cat#AM1340 Picoliter microinjection system WARNER Cat#PLI-100A Fast RNA-seq Lib Prep Kit V2 ABclonal Cat#RK20306 RNeasy Micro Kit QIAGEN Cat#4004 VAHTS universal V8 RNA-seq library Prep Kit Vazyme Cat#NR605 SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian Takara-Clontech Cat#634413 Hyperactive Universal CUT&Tag Assay Kit for Illumina Vazyme Cat#TD903 RNA clean&ConcentratorTM-5 kit Zymo Cat#R1014 Deposited data wild type_hESCs_rep1 This study GSE266418 wild type_hESCs_rep2 This study GSE266418 IGF2BP1-KO_hESCs_rep1 This study GSE266418 IGF2BP1-KO_hESCs_rep2 This study GSE266418 OTX2-KO_hESCs_rep1 This study GSE266418 OTX2-KO_hESCs_rep2 This study GSE266418 OTX2-GFP-OE_hESCs_rep1 This study GSE266418 OTX2-GFP-OE_hESCs_rep2 This study GSE266418 wild type_endoderm_rep1 This study GSE266418 wild type_endoderm_rep2 This study GSE266418 IGF2BP1-KO_endoderm_rep1 This study GSE266418 IGF2BP1-KO_endoderm_rep2 This study GSE266418 wild type_mesoderm_rep1 This study GSE266418 wild type_mesoderm_rep2 This study GSE266418 IGF2BP1-KO_mesoderm_rep1 This study GSE266418 IGF2BP1-KO_mesoderm_rep2 This study GSE266418 wild type_ectoderm_rep1 This study GSE266418 wild type_ectoderm_rep2 This study GSE266418 IGF2BP1-KO_ectoderm_rep1 This study GSE266418 IGF2BP1-KO_ectoderm_rep2 This study GSE266418 wild type_iMeLCs_rep1 This study GSE266418 wild type_iMeLCs_rep2 This study GSE266418 IGF2BP1-KO_iMeLCs_rep1 This study GSE266418 (Continued on next page) e3 Cell Stem Cell 32, 1–17.e1–e13, July 3, 2025
Magnetic Beads:Article Title: IGF2BP1 restricts the induction of human primordial germ cell fate in an m 6 A-dependent manner.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Matrigel Corning Cat#354234 Nuwacell® ncTarget hPSC Medium Nuwacell Cat#RP01020 Primocin InvitroGen Cat#ant-pm-2 Dispase STEMCELL TM Technologies Cat#07923 GibcoTM BASIC DMEM/F-12, HEPES GIBCO CatC11330500BT KnockOut Serum GIBCO Cat#10828028 non-essential amino acids GIBCO Cat#11140-050 β-mercaptoethanol GIBCO Cat#21985023 Penicillin-Streptomycin-Glutamine Invitrogen Cat#10378016 ACCUTASE STEMCELL TM Technologies Cat#07992 Human plasma fibronectin Invitrogen Cat#33016015 sodium pyruvate GIBCO Cat#11360070 FGF2 Peprotech Cat#100-18B ACTA Peprotech Cat#AF120-14E CHIR99021 Stemgent Cat#04000410 Y-27632 (Selleck, S1049) Selleck Cat#S1049 Glasgow’s MEM GIBCO Cat#11710-035 Anti-Adherence Rinsing Solution STEMCELL TM Technologies Cat#07010 LIF PeproTech Cat#300-05-25 BMP4 R&D Systems Cat#314-BP-01M EGF R&D Systems Cat#236-EG-01M T4 enzyme New England Biolabs Cat#M0202L BbSI enzyme New England Biolabs Cat#R3539 Puromycin Selleck Cat#S7417 Blasticidin Solarbio Cat#B9300 Ganciclovir MCE Cat#82410-32-0 PEI Polysciences Cat#24765100 PEG 8000 Solarbio Cat#P8260 0.05% trypsin GIBCO Cat#25300054 7AAD Beyotime Cat#ST515 4% paraformaldehyde Biosharp Cat#BL539A DAPI Beyotime Cat#C1002 AG RNAex Pro Reagent Accurate Biology Cat#AG21101 Actinomycin D Sigma-Aldrich Cat#SBR00013 RNasin Plus RNase Inhibitor Promega Cat#N2611 Cocktail protease inhibitors Roche N/A Protein A/G magnetic beads Thermo Fisher Scientific Cat#88802 FLAG M2 magnetic beads Sigma-Aldrich Cat#A2220 Proteinase K Thermo Fisher Scientific Cat#EO0491 TRIzol Invitrogen Cat#15596026 VAHTS mRNA Capture Beads Vazyme Cat#N401 IGEPAL® CA-630 Sigma-Aldrich Cat#56741 Protease inhibitors Sigma-Aldrich Cat#1187358001 Iodoacetamide Sigma-Aldrich Cat#I1149 Trypsin Promega Cat#V5280 TFA Sigma-Aldrich Cat#1002641000 RIPA buffer Beyotime Cat#P0013C Aprotinin Solarbio Cat#IA0430 Leupeptin Solarbio Cat#IL0070 (Continued on next page) Cell Stem Cell 32, 1–17.e1–e13, July 3, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Pepstatin Solarbio Cat#IP0170 HA beads Smart-Lifesciences Cat#SA068001 Ni-charged magnetic beads Engibody Cat#IF9301 nuclease P1 Wako Cat#145-08221 Shrimp alkaline phosphatase NEB Cat#M0371V Critical commercial assays STEMdiff TM Mesoderm Induction Medium Kit STEMCELL TM Technologies Cat#05220 STEMdiff TM Definitive Endoderm Medium Kit STEMCELL TM Technologies Cat#05110 STEMdiff TM Neural induction medium Kit STEMCELL TM Technologies Cat#08581 Phanta Max Super-Fidelity DNA Polymerase Vazyme Cat#PD505 ClonExpress MultiS One Step Cloning Kit Vazyme Cat#C11302 ClonExpressII One Step Cloning Kit Vazyme Cat#C11202 P3 Primary Cell 4D-Nucleofector X Kit Lonza Cat#V4XP-3024 EdU assay kit Sangon Biotech Cat#E6072040100 Dual Luciferase Reporter Gene Assay Kit Beyotime Cat#RG028 Western-Bright ECL kit Advansta Cat#K12045D50 mMESSAGE mMACHINE® SP6 Kit Invitrogen/ambion Cat#AM1340 Picoliter microinjection system WARNER Cat#PLI-100A Fast RNA-seq Lib Prep Kit V2 ABclonal Cat#RK20306 RNeasy Micro Kit QIAGEN Cat#4004 VAHTS universal V8 RNA-seq library Prep Kit Vazyme Cat#NR605 SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian Takara-Clontech Cat#634413 Hyperactive Universal CUT&Tag Assay Kit for Illumina Vazyme Cat#TD903 RNA clean&ConcentratorTM-5 kit Zymo Cat#R1014 Deposited data wild type_hESCs_rep1 This study GSE266418 wild type_hESCs_rep2 This study GSE266418 IGF2BP1-KO_hESCs_rep1 This study GSE266418 IGF2BP1-KO_hESCs_rep2 This study GSE266418 OTX2-KO_hESCs_rep1 This study GSE266418 OTX2-KO_hESCs_rep2 This study GSE266418 OTX2-GFP-OE_hESCs_rep1 This study GSE266418 OTX2-GFP-OE_hESCs_rep2 This study GSE266418 wild type_endoderm_rep1 This study GSE266418 wild type_endoderm_rep2 This study GSE266418 IGF2BP1-KO_endoderm_rep1 This study GSE266418 IGF2BP1-KO_endoderm_rep2 This study GSE266418 wild type_mesoderm_rep1 This study GSE266418 wild type_mesoderm_rep2 This study GSE266418 IGF2BP1-KO_mesoderm_rep1 This study GSE266418 IGF2BP1-KO_mesoderm_rep2 This study GSE266418 wild type_ectoderm_rep1 This study GSE266418 wild type_ectoderm_rep2 This study GSE266418 IGF2BP1-KO_ectoderm_rep1 This study GSE266418 IGF2BP1-KO_ectoderm_rep2 This study GSE266418 wild type_iMeLCs_rep1 This study GSE266418 wild type_iMeLCs_rep2 This study GSE266418 IGF2BP1-KO_iMeLCs_rep1 This study GSE266418 (Continued on next page) e3 Cell Stem Cell 32, 1–17.e1–e13, July 3, 2025
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